{"id":1193,"date":"2026-05-24T15:47:07","date_gmt":"2026-05-24T15:47:07","guid":{"rendered":"https:\/\/scientificadvances.org\/?p=1193"},"modified":"2026-05-24T15:47:07","modified_gmt":"2026-05-24T15:47:07","slug":"flow-cytometric-analysis-of-cell-surface-proteins-demonstrated-that-the-cells-were-cd29-and-cd90-positive-and-mhc-class-i-low-and-were-negative-for-mhc-class-ii-cd45-cd106-and-the-costi","status":"publish","type":"post","link":"https:\/\/scientificadvances.org\/?p=1193","title":{"rendered":"\ufeffFlow cytometric analysis of cell-surface proteins demonstrated that the cells were CD29- and CD90-positive, and MHC class I low, and were negative for MHC class II, CD45, CD106, and the costimulatory molecules CD80 and CD86"},"content":{"rendered":"

\ufeffFlow cytometric analysis of cell-surface proteins demonstrated that the cells were CD29- and CD90-positive, and MHC class I low, and were negative for MHC class II, CD45, CD106, and the costimulatory molecules CD80 and CD86. effects were associated with MAPC-induced prevention of hypomyelination after global HI. Besides attenuation of the cerebral inflammatory response, our findings showed that MAPC cells modulated the peripheral splenic inflammatory response, which has been implicated in the etiology of hypoxic-ischemic injury in the preterm brain. == Conclusions == In a pre-clinical animal model MAPC cell therapy improved the functional and structural outcome of the preterm brain after global HI. Future studies should establish the mechanism and long-term therapeutic effects of neuroprotection established by MAPC cells in the developing preterm brain exposed to HI. Our study may form the basis for future clinical trials, which will evaluate whether MAPC therapy is capable of reducing neurological sequelae in preterm infants with hypoxic-ischemic encephalopathy. Keywords: Preterm, Hypoxic-ischemic encephalopathy, Multipotent adult progenitor cells, Neuroprotection == Background == Hypoxia-ischemia (HI) in the developing brain is strongly correlated with mortality and neurological morbidity in preterm and full-term infants, resulting in an enormous physical, psychological, and economic burden [1]. Unfortunately, no therapeutic cure is available for hypoxic-ischemic brain injury in preterm infants. In a translational animal model of global HI in the preterm ovine fetus, our group has demonstrated that cell-based therapy may be a promising neuroprotective strategy for preterm AZ 10417808 neonates suffering from HI-induced brain injury [2]. We showed that intravenous administration of exogenous mesenchymal stem cells (MSC) protected against functional loss and structural injury of the preterm brain [2]. These neuroprotective effects were largely attributable to attenuation of (neuro) inflammatory processes, as indicated by decreased microglial activation and proliferation in the preterm brain and induction of peripheral T cell tolerance, which was associated with reduced cerebral infiltration of these immune effector cells [2]. Multipotent Rabbit polyclonal to HOXA1<\/a> adult progenitor cells (MAPC), which are adherent bone marrow-derived cells of an earlier developmental stage than MSC have a high expansion potential, and their immunological properties make it possible to use them as a universal allogeneic donor [35]. Pre-clinical animal studies have demonstrated that in comparison to MSC, MAPC cells have stronger anti-inflammatory effects and are more AZ 10417808 potent in promoting endogenous tissue regeneration after ischemic and traumatic injury to the CNS [611]. In addition AZ 10417808<\/a> , the smaller size of MAPC cells compared to MSC facilitates passage through the pulmonary capillary bed, which may increase availability of MAPC cells in the systemic and cerebral vasculature and thus enhancing their neuroprotective effect [12]. Based on these superior qualities of MAPC, we aimed to assess the anti-inflammatory and neuroprotective potential of MAPC cells in the preterm brain exposed to global HI. We hypothesized that systemic administration of MAPC cells would attenuate cerebral and peripheral inflammation and prevent structural and functional brain injury after global HI in the preterm ovine fetus. We tested our hypothesis in a well-established pre-clinical animal model of preterm global HI. In this model, preterm ovine fetuses were exposed to global HI by transient umbilical cord occlusion (UCO) at 0. 7 gestation which is equivalent to 3032 weeks human gestation [13] followed by systemic administration of MAPC therapy during AZ 10417808 a 7-day reperfusion period. The anti-inflammatory effects of MAPC cells were assessed in the preterm brain by analysis of microglial responses in the hippocampus and subcortical white matter. The spleen was assessed, since previous reports suggest that MAPC-induced alterations in the splenic inflammatory response may be responsible for their neuroprotective effect after brain injury [9]. Structural effects of systemic MAPC administration were assessed by histological white matter injury examination. Brain function was studied by analysis of cortical function by means of electrographic seizure activity and central (brain stem) function by means of baroreflex sensitivity. == Methods == == Study approval == The experimental protocol and study design AZ 10417808 were in line with the institutional guides for animal experiments and were approved by the institutional Animal Ethics Research committee of Maastricht University, The Netherlands. == Randomization and blinding == Thirty-two singleton fetuses of time-mated Texel ewes were randomized by an independent researcher who was not involved in the animal experiments. Randomization resulted in four experimental groups: (1) sham umbilical cord occlusion, saline treatment (sham-SALn= 8), (2) sham umbilical cord occlusion, MAPC treatment (sham-MAPC; n= 8), (3) umbilical.<\/p>\n","protected":false},"excerpt":{"rendered":"

\ufeffFlow cytometric analysis of cell-surface proteins demonstrated that the cells were CD29- and CD90-positive, and MHC class I low, and were negative for MHC class II, CD45, CD106, and the costimulatory molecules CD80 and CD86. effects were associated with MAPC-induced prevention of hypomyelination after global HI. Besides attenuation of the cerebral inflammatory response, our findings […]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[14],"tags":[],"class_list":["post-1193","post","type-post","status-publish","format-standard","hentry","category-non-selective-cck"],"_links":{"self":[{"href":"https:\/\/scientificadvances.org\/index.php?rest_route=\/wp\/v2\/posts\/1193","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/scientificadvances.org\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/scientificadvances.org\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/scientificadvances.org\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/scientificadvances.org\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1193"}],"version-history":[{"count":1,"href":"https:\/\/scientificadvances.org\/index.php?rest_route=\/wp\/v2\/posts\/1193\/revisions"}],"predecessor-version":[{"id":1194,"href":"https:\/\/scientificadvances.org\/index.php?rest_route=\/wp\/v2\/posts\/1193\/revisions\/1194"}],"wp:attachment":[{"href":"https:\/\/scientificadvances.org\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1193"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/scientificadvances.org\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=1193"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/scientificadvances.org\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=1193"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}